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gtpase 13b  (Proteintech)


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    Structured Review

    Proteintech gtpase 13b
    Gtpase 13b, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 886 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gtpase 13b/product/Proteintech
    Average 96 stars, based on 886 article reviews
    gtpase 13b - by Bioz Stars, 2026-02
    96/100 stars

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    Proteintech primary antibodies against adp ribosylation factor like gtpase 13b arl13b
    Figure 1. Cilia patterning in pituitary neuroendocrine tumors (PitNETs) and in healthy pituitary. Clinical and pathological characteristics of PitNET patients (A). Representative images captured using confocal microscopy characterizing the primary cilia (PC) of controls and PitNETs stained with <t>Arl13b</t> (in red) and nuclei counterstained with 40,6-diamidino-2- phenylindole (DAPI; blue). Scale bar ¼ 50 mm and 10 mm (B). Representative images of the different phenotypes found in PitNET tissues: dot-like and normal cilia (C). Violin plot displaying the quantification of PC using the cilia index score, as reported in the Material and Methods section (D). Receiver operating characteristic (ROC) curve analyses performed to assess the diagnostic value of CI to discriminate between healthy subjects and patients (controls vs PitNETs) (E). The table shows values of cutoff, area under the curve (AUC), 95% CIs, sensitivity, and specificity of the ROC curve analyses for CI (F). Data correspond to the median (line within violin). Differences were analyzed using the Mann-Whitney test. ns, not significant; ****P < .001.
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    Figure 1. Cilia patterning in pituitary neuroendocrine tumors (PitNETs) and in healthy pituitary. Clinical and pathological characteristics of PitNET patients (A). Representative images captured using confocal microscopy characterizing the primary cilia (PC) of controls and PitNETs stained with <t>Arl13b</t> (in red) and nuclei counterstained with 40,6-diamidino-2- phenylindole (DAPI; blue). Scale bar ¼ 50 mm and 10 mm (B). Representative images of the different phenotypes found in PitNET tissues: dot-like and normal cilia (C). Violin plot displaying the quantification of PC using the cilia index score, as reported in the Material and Methods section (D). Receiver operating characteristic (ROC) curve analyses performed to assess the diagnostic value of CI to discriminate between healthy subjects and patients (controls vs PitNETs) (E). The table shows values of cutoff, area under the curve (AUC), 95% CIs, sensitivity, and specificity of the ROC curve analyses for CI (F). Data correspond to the median (line within violin). Differences were analyzed using the Mann-Whitney test. ns, not significant; ****P < .001.
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    Proteintech ciliary component adp ribosylation factor like gtpase 13b
    Figure 1. Cilia patterning in pituitary neuroendocrine tumors (PitNETs) and in healthy pituitary. Clinical and pathological characteristics of PitNET patients (A). Representative images captured using confocal microscopy characterizing the primary cilia (PC) of controls and PitNETs stained with <t>Arl13b</t> (in red) and nuclei counterstained with 40,6-diamidino-2- phenylindole (DAPI; blue). Scale bar ¼ 50 mm and 10 mm (B). Representative images of the different phenotypes found in PitNET tissues: dot-like and normal cilia (C). Violin plot displaying the quantification of PC using the cilia index score, as reported in the Material and Methods section (D). Receiver operating characteristic (ROC) curve analyses performed to assess the diagnostic value of CI to discriminate between healthy subjects and patients (controls vs PitNETs) (E). The table shows values of cutoff, area under the curve (AUC), 95% CIs, sensitivity, and specificity of the ROC curve analyses for CI (F). Data correspond to the median (line within violin). Differences were analyzed using the Mann-Whitney test. ns, not significant; ****P < .001.
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    Image Search Results


    Figure 1. Cilia patterning in pituitary neuroendocrine tumors (PitNETs) and in healthy pituitary. Clinical and pathological characteristics of PitNET patients (A). Representative images captured using confocal microscopy characterizing the primary cilia (PC) of controls and PitNETs stained with Arl13b (in red) and nuclei counterstained with 40,6-diamidino-2- phenylindole (DAPI; blue). Scale bar ¼ 50 mm and 10 mm (B). Representative images of the different phenotypes found in PitNET tissues: dot-like and normal cilia (C). Violin plot displaying the quantification of PC using the cilia index score, as reported in the Material and Methods section (D). Receiver operating characteristic (ROC) curve analyses performed to assess the diagnostic value of CI to discriminate between healthy subjects and patients (controls vs PitNETs) (E). The table shows values of cutoff, area under the curve (AUC), 95% CIs, sensitivity, and specificity of the ROC curve analyses for CI (F). Data correspond to the median (line within violin). Differences were analyzed using the Mann-Whitney test. ns, not significant; ****P < .001.

    Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

    Article Title: Primary Cilia as a Tumor Marker in Pituitary Neuroendocrine Tumors.

    doi: 10.1016/j.modpat.2024.100475

    Figure Lengend Snippet: Figure 1. Cilia patterning in pituitary neuroendocrine tumors (PitNETs) and in healthy pituitary. Clinical and pathological characteristics of PitNET patients (A). Representative images captured using confocal microscopy characterizing the primary cilia (PC) of controls and PitNETs stained with Arl13b (in red) and nuclei counterstained with 40,6-diamidino-2- phenylindole (DAPI; blue). Scale bar ¼ 50 mm and 10 mm (B). Representative images of the different phenotypes found in PitNET tissues: dot-like and normal cilia (C). Violin plot displaying the quantification of PC using the cilia index score, as reported in the Material and Methods section (D). Receiver operating characteristic (ROC) curve analyses performed to assess the diagnostic value of CI to discriminate between healthy subjects and patients (controls vs PitNETs) (E). The table shows values of cutoff, area under the curve (AUC), 95% CIs, sensitivity, and specificity of the ROC curve analyses for CI (F). Data correspond to the median (line within violin). Differences were analyzed using the Mann-Whitney test. ns, not significant; ****P < .001.

    Article Snippet: Primary antibodies against ADP ribosylation factor-like GTPase 13B (Arl13B) (Proteintech Cat# 17711-1-AP, RRID:AB_2060867), GH (R and D Systems Cat# AF1067, RRID:AB_354573), PRL (Santa Cruz Biotechnology Cat# sc-46698, RRID:AB_628174), FSH (Ventana Medical Systems Cat# 760-2710, RRID:AB_2335963), ACTH (Santa Cruz Biotechnology Cat# sc-69648, RRID:AB_1118657), and LH (Ventana Medical Systems Cat# 760-2802 RRID:AB_2939029) were incubated overnight at 4 C. The following day, an Alexa 488- labeled donkey antimouse (Thermo Fisher Scientific Cat# A32766, RRID:AB_2762823), an Alexa 568-labeled donkey antirabbit (Thermo Fisher Scientific Cat# A10042, RRID:AB_2534017), an Alexa 488-labeled donkey antigoat (Thermo Fisher Scientific Cat# A-11055, RRID:AB_2534102), and an Alexa 647-labeled donkey antigoat (Thermo Fisher Scientific Cat# A-21447, RRID:AB_2535864) were used as secondary antibodies, and nuclei were counterstained with 40,6-diamidino-2-phenylindole.

    Techniques: Confocal Microscopy, Staining, Diagnostic Assay, MANN-WHITNEY

    Figure 2. Cilia patterning of the different tumor types compared with controls. Representative images of double immunofluorescence staining for growth hormone (GH), prolactin (PRL), adrenocorticotroph hormone (ACTH), and follicle-stimulating (FSH; in green) and Arl13b (in red) in different pituitary neuroendocrine tumors (PitNETs) and in controls. Scale bar ¼ 50 mm and 10 mm (A). Box plots representing cilia index in the different types of PitNETs: Functioning versus nonfunctional pituitary neuroendocrine tumors (NF-PitNETs) (B) according to their pathological classification (C). Data corresponding to the median (lines within violins). Differences were analyzed using the KruskaleWallis test. *P < .05; **P < .01; ***P < .005; ****P < .001.

    Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

    Article Title: Primary Cilia as a Tumor Marker in Pituitary Neuroendocrine Tumors.

    doi: 10.1016/j.modpat.2024.100475

    Figure Lengend Snippet: Figure 2. Cilia patterning of the different tumor types compared with controls. Representative images of double immunofluorescence staining for growth hormone (GH), prolactin (PRL), adrenocorticotroph hormone (ACTH), and follicle-stimulating (FSH; in green) and Arl13b (in red) in different pituitary neuroendocrine tumors (PitNETs) and in controls. Scale bar ¼ 50 mm and 10 mm (A). Box plots representing cilia index in the different types of PitNETs: Functioning versus nonfunctional pituitary neuroendocrine tumors (NF-PitNETs) (B) according to their pathological classification (C). Data corresponding to the median (lines within violins). Differences were analyzed using the KruskaleWallis test. *P < .05; **P < .01; ***P < .005; ****P < .001.

    Article Snippet: Primary antibodies against ADP ribosylation factor-like GTPase 13B (Arl13B) (Proteintech Cat# 17711-1-AP, RRID:AB_2060867), GH (R and D Systems Cat# AF1067, RRID:AB_354573), PRL (Santa Cruz Biotechnology Cat# sc-46698, RRID:AB_628174), FSH (Ventana Medical Systems Cat# 760-2710, RRID:AB_2335963), ACTH (Santa Cruz Biotechnology Cat# sc-69648, RRID:AB_1118657), and LH (Ventana Medical Systems Cat# 760-2802 RRID:AB_2939029) were incubated overnight at 4 C. The following day, an Alexa 488- labeled donkey antimouse (Thermo Fisher Scientific Cat# A32766, RRID:AB_2762823), an Alexa 568-labeled donkey antirabbit (Thermo Fisher Scientific Cat# A10042, RRID:AB_2534017), an Alexa 488-labeled donkey antigoat (Thermo Fisher Scientific Cat# A-11055, RRID:AB_2534102), and an Alexa 647-labeled donkey antigoat (Thermo Fisher Scientific Cat# A-21447, RRID:AB_2535864) were used as secondary antibodies, and nuclei were counterstained with 40,6-diamidino-2-phenylindole.

    Techniques: Staining

    Figure 6. Schematic stages of ciliogenesis in pituitary neuroendocrine tumors (PitNETs). Model of the different stages of intracellular ciliogenesis found in PitNETs, including key molecular steps. At the first steps, preciliary vesicles (CVs) associate with the distal appendages of the mother centriole. The assembly and fusion of CVs requires a SNARE complex in cooperation with SNAP25 and STX3. EHD1-3 proteins mediate the fusion to a larger vesicle. In the next steps, the ciliation inhibitor CCP110 is removed and Arl13b and Rab8 assemble to the ciliary membrane regulating the growth of the axoneme (adapted from Zhao et al and Breslow and Holland56,57).

    Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

    Article Title: Primary Cilia as a Tumor Marker in Pituitary Neuroendocrine Tumors.

    doi: 10.1016/j.modpat.2024.100475

    Figure Lengend Snippet: Figure 6. Schematic stages of ciliogenesis in pituitary neuroendocrine tumors (PitNETs). Model of the different stages of intracellular ciliogenesis found in PitNETs, including key molecular steps. At the first steps, preciliary vesicles (CVs) associate with the distal appendages of the mother centriole. The assembly and fusion of CVs requires a SNARE complex in cooperation with SNAP25 and STX3. EHD1-3 proteins mediate the fusion to a larger vesicle. In the next steps, the ciliation inhibitor CCP110 is removed and Arl13b and Rab8 assemble to the ciliary membrane regulating the growth of the axoneme (adapted from Zhao et al and Breslow and Holland56,57).

    Article Snippet: Primary antibodies against ADP ribosylation factor-like GTPase 13B (Arl13B) (Proteintech Cat# 17711-1-AP, RRID:AB_2060867), GH (R and D Systems Cat# AF1067, RRID:AB_354573), PRL (Santa Cruz Biotechnology Cat# sc-46698, RRID:AB_628174), FSH (Ventana Medical Systems Cat# 760-2710, RRID:AB_2335963), ACTH (Santa Cruz Biotechnology Cat# sc-69648, RRID:AB_1118657), and LH (Ventana Medical Systems Cat# 760-2802 RRID:AB_2939029) were incubated overnight at 4 C. The following day, an Alexa 488- labeled donkey antimouse (Thermo Fisher Scientific Cat# A32766, RRID:AB_2762823), an Alexa 568-labeled donkey antirabbit (Thermo Fisher Scientific Cat# A10042, RRID:AB_2534017), an Alexa 488-labeled donkey antigoat (Thermo Fisher Scientific Cat# A-11055, RRID:AB_2534102), and an Alexa 647-labeled donkey antigoat (Thermo Fisher Scientific Cat# A-21447, RRID:AB_2535864) were used as secondary antibodies, and nuclei were counterstained with 40,6-diamidino-2-phenylindole.

    Techniques: Membrane