Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
Article Title: Primary Cilia as a Tumor Marker in Pituitary Neuroendocrine Tumors.
doi: 10.1016/j.modpat.2024.100475
Figure Lengend Snippet: Figure 1. Cilia patterning in pituitary neuroendocrine tumors (PitNETs) and in healthy pituitary. Clinical and pathological characteristics of PitNET patients (A). Representative images captured using confocal microscopy characterizing the primary cilia (PC) of controls and PitNETs stained with Arl13b (in red) and nuclei counterstained with 40,6-diamidino-2- phenylindole (DAPI; blue). Scale bar ¼ 50 mm and 10 mm (B). Representative images of the different phenotypes found in PitNET tissues: dot-like and normal cilia (C). Violin plot displaying the quantification of PC using the cilia index score, as reported in the Material and Methods section (D). Receiver operating characteristic (ROC) curve analyses performed to assess the diagnostic value of CI to discriminate between healthy subjects and patients (controls vs PitNETs) (E). The table shows values of cutoff, area under the curve (AUC), 95% CIs, sensitivity, and specificity of the ROC curve analyses for CI (F). Data correspond to the median (line within violin). Differences were analyzed using the Mann-Whitney test. ns, not significant; ****P < .001.
Article Snippet: Primary antibodies against ADP ribosylation factor-like GTPase 13B (Arl13B) (Proteintech Cat# 17711-1-AP, RRID:AB_2060867), GH (R and D Systems Cat# AF1067, RRID:AB_354573), PRL (Santa Cruz Biotechnology Cat# sc-46698, RRID:AB_628174), FSH (Ventana Medical Systems Cat# 760-2710, RRID:AB_2335963), ACTH (Santa Cruz Biotechnology Cat# sc-69648, RRID:AB_1118657), and LH (Ventana Medical Systems Cat# 760-2802 RRID:AB_2939029) were incubated overnight at 4 C. The following day, an Alexa 488- labeled donkey antimouse (Thermo Fisher Scientific Cat# A32766, RRID:AB_2762823), an Alexa 568-labeled donkey antirabbit (Thermo Fisher Scientific Cat# A10042, RRID:AB_2534017), an Alexa 488-labeled donkey antigoat (Thermo Fisher Scientific Cat# A-11055, RRID:AB_2534102), and an Alexa 647-labeled donkey antigoat (Thermo Fisher Scientific Cat# A-21447, RRID:AB_2535864) were used as secondary antibodies, and nuclei were counterstained with 40,6-diamidino-2-phenylindole.
Techniques: Confocal Microscopy, Staining, Diagnostic Assay, MANN-WHITNEY